Measurement of enzymatic activities in turbid suspensions by double beam spectrophotometry

Autor:	R, Garcia-Cañero, M, Gosálvez
Rok vydání:	1976
Předmět:	Spectrophotometry Pyruvate Kinase Animals Mitochondria Liver Amidohydrolases Nicotinamidase Rats
Zdroj:	Revista espanola de fisiologia. 32(4)
ISSN:	0034-9402
Popis:	A method for the determination of enzyme activities in turbid suspension is described, based on the use of a double beam or double wavelength spectrophotometer in one or two cell mode. It measures the disappearance of NADH, coupled to the activity of the enzyme, at a 340 wavelength or at a 340 minus 374 mmu wavelength pair. The method has been applied to the determination of pyruvate kinase activity, in the presence of isolated mitochondria, and other possible applications are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::7bd2f9b105c9e08c23bc11293ffa11ac https://pubmed.ncbi.nlm.nih.gov/1005887 Zobrazit plný text záznamu