| Autor: |
Dong-na, Li, You-sheng, Liang, Yong-hua, Zhou, Huan-xiang, Zhang, Hai-ying, Sheng, Wei, Luo, Wei, Gong, Hong-xiang, Zhuge |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitologyparasitic diseases. 28(4) |
| ISSN: |
1000-7423 |
| Popis: |
Purified astrocytes were cultured in plates. When astrocytes grew over 80% of the plate, tachyzoites of Toxoplasma gondii RH strain were added for co-culture. In the period of 0-72 h, change of the astrocytes and tachyzoites was observed after Giemsa staining. In 0-48 h, monodansylcadaverine (MDC) was used to study the action of autophagy in the process of tachyzoites invading astrocytes. At 1 h co-culture, tachyzoites had entered in astrocytes and the autophagosomes appeared. At 4 h, the autophagosomes increased pronouncedly. However, after 12 h, number of autophagosomes considerably decreased and damage of the cells occurred. 48 h later, autophagosomes disappeared and more astrocytes were destroyed. At 72 h most cells destroyed and tachyzoites were released. The result showed that autophagy is inhibited when the astrocytes were in vitro infected by tachyzoites. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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