Melatonin Binding to Human NQO2 by Isothermal Titration Calorimetry
Autor: | Barbara, Calamini, Gilles, Ferry, Jean A, Boutin |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Methods in molecular biology (Clifton, N.J.). 2550 |
ISSN: | 1940-6029 |
Popis: | To ensure the physical interaction between a protein and its ligand, many techniques can be applied. One of them, isothermal titration calorimetry (ITC), measures the heat exchange between a forming molecular complex and its milieu. From this heat exchange, it is possible to acquire the thermodynamic parameters, the binding stoichiometry and the affinity constant (K |
Databáze: | OpenAIRE |
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