| Autor: |
Ia T, Terletskaia, L P, Syrovatskaia, E P, Khzuliná, M N, Ovander, Ia V, Belik |
| Rok vydání: |
1980 |
| Předmět: |
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| Zdroj: |
Ukrainskii biokhimicheskii zhurnal (1. 52(6) |
| ISSN: |
0201-8470 |
| Popis: |
Protein SCP is found in myelin of spinal cord and spinal roots. It is shown that its amount accounts for 12% of the total protein content in myelin of spinal roots and only for 2% in myelin of spinal cord. Almost all the studied protein is extracted from myelin with 0.1 M NaCl (80-90%). The absolute identity of protens SCP and P2 is established using the cross reaction immunodiffusion with monospecific antisera. It is shown that- N-terminal amino acid in protein SCP, like in protein P2 is blocked. On the basis of the data obtained a conclusion is made that protein P2 is not an integral protein of myelin. However, myelin is capable under conditions of a nonionic medium of binding protein which then may be easily extracted by increasing the medium ionic strength. This gave reasons to propose a method for protein P2 isolation from myelin using 0.15 M NaCl with the subsequent purification by means of Sephadex G-50 gelfiltration. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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