Proteomic analysis of pancreatic secretory trypsin inhibitor/tumor-associated trypsin inhibitor from urine of patients with pancreatitis or prostate cancer

Autor:	Leena, Valmu, Suvi, Ravela, Ulf-Håkan, Stenman
Rok vydání:	2010
Předmět:	Male Proteomics Spectrometry Mass Electrospray Ionization Pancreatitis Trypsin Inhibitor Kazal Pancreatic Analytic Sample Preparation Methods Humans Prostatic Neoplasms Urinalysis Carrier Proteins Peptides Trypsin Inhibitors Chromatography Liquid
Zdroj:	Methods in molecular biology (Clifton, N.J.). 641
ISSN:	1940-6029
Popis:	The development of proteomic methods, especially mass spectrometry, has brought new possibilities to tumor marker research. Pancreatic secretory trypsin inhibitor (PSTI), a common known biomarker for various malignancies, occurs on genetic variants that we are able to detect at the protein level with proteomic techniques using immunoaffinity capture prior to liquid chromatography-mass spectrometry (LC-MS). We also show that PSTI can be detected in urine from cancer patients using a two-step peptide enrichment technique and LC-MS. These results show that tumor-associated peptides can be detected in urine by proteomic techniques.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::7b2b74dbcea4de5b2681a83b129171f1 https://pubmed.ncbi.nlm.nih.gov/20407956 Zobrazit plný text záznamu