Autor: |
Leena, Valmu, Suvi, Ravela, Ulf-Håkan, Stenman |
Rok vydání: |
2010 |
Předmět: |
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Zdroj: |
Methods in molecular biology (Clifton, N.J.). 641 |
ISSN: |
1940-6029 |
Popis: |
The development of proteomic methods, especially mass spectrometry, has brought new possibilities to tumor marker research. Pancreatic secretory trypsin inhibitor (PSTI), a common known biomarker for various malignancies, occurs on genetic variants that we are able to detect at the protein level with proteomic techniques using immunoaffinity capture prior to liquid chromatography-mass spectrometry (LC-MS). We also show that PSTI can be detected in urine from cancer patients using a two-step peptide enrichment technique and LC-MS. These results show that tumor-associated peptides can be detected in urine by proteomic techniques. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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