Decellularized extracellular matrix microparticles as a vehicle for cellular delivery in a model of anastomosis healing

Autor:	David M, Hoganson, Gwen E, Owens, Amanda M, Meppelink, Erik K, Bassett, Chris M, Bowley, Cameron J, Hinkel, Eric B, Finkelstein, Scott M, Goldman, Joseph P, Vacanti
Rok vydání:	2015
Předmět:	Wound Healing Drug Delivery Systems Cell Movement Tensile Strength Anastomosis Surgical Sus scrofa Animals Humans Collagen Fibroblasts Models Biological Microspheres Extracellular Matrix
Zdroj:	Journal of biomedical materials research. Part A. 104(7)
ISSN:	1552-4965
Popis:	Extracellular matrix (ECM) materials from animal and human sources have become important materials for soft tissue repair. Microparticles of ECM materials have increased surface area and exposed binding sites compared to sheet materials. Decellularized porcine peritoneum was mechanically dissociated into 200 µm microparticles, seeded with fibroblasts and cultured in a low gravity rotating bioreactor. The cells avidly attached and maintained excellent viability on the microparticles. When the seeded microparticles were placed in a collagen gel, the cells quickly migrated off the microparticles and through the gel. Cells from seeded microparticles migrated to and across an in vitro anastomosis model, increasing the tensile strength of the model. Cell seeded microparticles of ECM material have potential for paracrine and cellular delivery therapies when delivered in a gel carrier. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1728-1735, 2016.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::7b1101329413cb741b3b5c3502d9078f https://pubmed.ncbi.nlm.nih.gov/26946064 Zobrazit plný text záznamu Plný text