| Autor: |
T, Peris Conejero, R, Olivares Pallerols, J L, Moreno Frigols |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
Revista espanola de medicina nuclear. 28(2) |
| ISSN: |
0212-6982 |
| Popis: |
immunoradiometric assay (IRMA) is one of the principal methods used for the analytical determination of neuron specific enolase (NSE) concentration. We studied the influence of temperature on the calibration curves obtained by this method, and a physicochemical justification based on two theoretical models is proposed.we used a commercially available RIA kit for NSE and a gamma counter. Data was analysed using Statistical software.activity bound to the antibody increases with temperature, producing results that are consistent with two modifications to the four parameter and Langmuir equations.the two models used successfully reproduce the results, with the adsorption model being preferable due to its greater simplicity and clearer physical significance. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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