BCL6 can repress transcription from the human immunodeficiency virus type I promoter/enhancer region
| Autor: | B W, Baron, M, Desai, L J, Baber, L, Paras, Q, Zhang, A, Sadhu, S, Duguay, G, Nucifora, T W, McKeithan, N, Zeleznik-Le |
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| Rok vydání: | 1997 |
| Předmět: |
Gene Expression Regulation
Viral Binding Sites DNA Complementary HIV Enhancer Base Sequence Transcription Genetic Blotting Western Molecular Sequence Data Zinc Fingers DNA DNA-Binding Proteins Genes Reporter Proto-Oncogene Proteins COS Cells Consensus Sequence HIV-1 Proto-Oncogene Proteins c-bcl-6 Animals Humans Electrophoresis Polyacrylamide Gel Luciferases Promoter Regions Genetic HeLa Cells Transcription Factors |
| Zdroj: | Genes, chromosomescancer. 19(1) |
| ISSN: | 1045-2257 |
| Popis: | The gene BCL6 encodes a zinc finger protein with similarities to transcription factors. We previously reported that a number of viral genomes, including human immunodeficiency virus type I (HIV-1), contain sequences which are similar to the BCL6 DNA-binding consensus in their promoter regions. Electrophoretic mobility shift assays showed that the full-length BCL6 protein extracted from transfected COS cells and a bacterially expressed truncated protein containing the BCL6 zinc fingers can bind specifically to DNA from the U3 promoter/enhancer region of HIV-1. Transient transfections were performed to analyze the effects of the BCL6 protein on luciferase expression driven by the HIV-1 long terminal repeat (LTR) sequences. Full-length BCL6 significantly repressed luciferase activity compared with multiple controls. We conclude that the BCL6 protein can bind to the HIV-1 promoter-enhancer region and contains a domain upstream of its zinc fingers that can repress transcription from the HIV-1 LTR. |
| Databáze: | OpenAIRE |
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