[The isolation of nucleases Sm1 and Sm2 in preparative amounts and a study of their antigenicity]

Autor:	M N, Filimonova, N G, Urazov, K S, Khaertynov
Rok vydání:	1992
Předmět:	Antigens Bacterial Endodeoxyribonucleases Endoribonucleases Immunization Secondary Animals Electrophoresis Polyacrylamide Gel Immunization Rabbits Immunoelectrophoresis Two-Dimensional Chromatography DEAE-Cellulose Serratia marcescens
Zdroj:	Nauchnye doklady vysshei shkoly. Biologicheskie nauki. (2)
ISSN:	0470-4606
Popis:	Two enzyme forms of endonuclease (Sm 1 and Sm 2) strain B10M1 in 60 and 100 mg respectively have been isolated from the culture fluid Serratia marcescens. The chromatographic and electrophoretic properties and N-terminal amino acid residues are different for both enzymes. The purification procedure consists of dialysis and ion-exchange chromatography on DEAE- and phosphocellulose. The yield of nucleases Sm1 and Sm2 are 14% and 28% respectively. The antigenic differences of nucleases Sm1 and Sm2 have been found by cross immunoenzyme analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::7ac0b39f45135430df36526dca2855b3 https://pubmed.ncbi.nlm.nih.gov/1302504 Zobrazit plný text záznamu