| Autor: |
József T, Tóth, Gergő, Gulyás, László, Hunyady, Péter, Várnai |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1949 |
| ISSN: |
1940-6029 |
| Popis: |
There are several difficulties to face when investigating the role of phosphoinositides. Although they are present in most organelles, their concentration is very low, sometimes undetectable with the available methods; moreover, their level can quickly change upon several external stimuli. Here we introduce a newly improved lipid sensor tool-set based on the balanced expression of luciferase-fused phosphoinositide recognizing protein domains and a Venus protein targeted to the plasma membrane, allowing us to perform Bioluminescence Resonance Energy Transfer (BRET) measurements that reflect phosphoinositide changes in a population of transiently transfected cells. This method is highly sensitive, specific, and capable of semiquantitative characterization of plasma membrane phosphoinositide changes with high temporal resolution. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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