Pax-6 interactions with TATA-box-binding protein and retinoblastoma protein
| Autor: | A, Cvekl, F, Kashanchi, J N, Brady, J, Piatigorsky |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Homeodomain Proteins
PAX6 Transcription Factor Molecular Sequence Data Epithelial Cells TATA-Box Binding Protein Crystallins Precipitin Tests Retinoblastoma Protein TATA Box Chromatography Affinity DNA-Binding Proteins Repressor Proteins Lens Crystalline Animals Paired Box Transcription Factors Amino Acid Sequence Rabbits Eye Proteins Cell Division Glutathione Transferase Plasmids Transcription Factors |
| Zdroj: | Investigative ophthalmologyvisual science. 40(7) |
| ISSN: | 0146-0404 |
| Popis: | To identify proteins that physically interact with Pax-6, a paired domain- and homeodomain (HD)-containing transcription factor that is a key regulator of eye development.Protein-protein interactions involving Pax-6, TATA-box-binding protein (TPB), and retinoblastoma protein were studied using affinity chromatography with Pax-6 as ligand, glutathione-S-transferase (GST) pull-down assays, and immunoprecipitations.The authors have shown that Pax-6 is a sequence-specific activator of many crystallin genes, all containing a TATA box, in the lens. Others have shown that lens fiber cell differentiation, characterized by temporally and spatially regulated crystallin gene expression, depends on retinoblastoma protein. In the present study it was shown that Pax-6 interacted with the TBP, the DNA-binding subunit of general transcription complex TFIID. GST pull-down assays indicated that this interaction was mediated by the Pax-6 HD, with a substantial role for its N-terminal arm and first two alpha-helices. The experiments also indicated a binding role for the C-terminal-activation domain of the protein. In addition, the present study showed that the HD of Pax-6 interacted with retinoblastoma protein. Immunoprecipitation experiments confirmed retinoblastoma protein/Pax-6 complexes in lens nuclear extracts.Blending the present results with those in the literature suggests that Pax-6 and retinoblastoma protein participate in overlapping regulatory pathways controlling epithelial cell division, fiber cell elongation, and crystallin gene expression during lens development. |
| Databáze: | OpenAIRE |
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