Autor: |
Jie-miao, Hu, Fei-chan, Qiu, Bin, Yin, Yan-hua, Gong, Jian-gang, Yuan, Bo-qin, Qiang, Shi-zhen, Wang, Xiao-zhong, Peng |
Rok vydání: |
2007 |
Předmět: |
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Zdroj: |
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. 29(3) |
ISSN: |
1000-503X |
Popis: |
To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin.We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character.We therefore examined clones of PC3 cells stably expressing fusion gene and positive controlfluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks.Noninvasive monitoring facilitates the detection of gene expression in vivo and in vitro. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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