Estimation of impurity profiles of drugs and related materials. 12. Isolation and identification of an isomeric impurity in danazol

Autor:	G, Balogh, E, Csizér, G G, Ferenczy, Z, Halmos, B, Herényi, P, Horváth, A, Laukó, S, Görög
Rok vydání:	1995
Předmět:	Chromatography Gas Magnetic Resonance Spectroscopy Isomerism Danazol Spectrophotometry Ultraviolet Chromatography Thin Layer Drug Contamination Chromatography High Pressure Liquid Densitometry
Zdroj:	Pharmaceutical research. 12(2)
ISSN:	0724-8741
Popis:	We report on a new isomeric impurity of danazol. This impurity designated as isodanazol was detected by reversed-phase high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC). Its structure was determined after separation by preparative HPLC. Mass spectrometry revealed the isomeric nature of the impurity while the UV spectrum indicated profound difference in the isoxazole moieties. The structure of the isomeric isoxazole ring in isodanazol was determined by NMR spectroscopy using COSY, HETCOR and NOE measurements. The difference between the UV spectra of danazol and isodanazol is explained on the basis of the difference between the aromaticities of their isoxazole rings supported by quantum chemical calculations. The quantitative determination of the impurity down to the 0.05% level can be performed by HPLC, gas chromatography and TLC densitometry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::7a4c8566b54e7331864564ae4a1206f7 https://pubmed.ncbi.nlm.nih.gov/7784348 Zobrazit plný text záznamu Full text from SpringerLink