Comparative genomics of ESBL-producing Escherichia coli (ESBL-Ec) reveals a similar distribution of the 10 most prevalent ESBL-Ec clones and ESBL genes among human community faecal and extra-intestinal infection isolates in the Netherlands (2014-17)
Autor: | T D, Verschuuren, D, van Hout, S, Arredondo-Alonso, A C, Fluit, E A, Reuland, J, Top, A C, Schürch, T, Bosch, M J M, Bonten, J A J W, Kluytmans, R J L, Willems |
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Rok vydání: | 2020 |
Předmět: |
Genotype
Genomics biochemical phenomena metabolism and nutrition bacterial infections and mycoses beta-Lactamases Anti-Bacterial Agents Clone Cells Feces AcademicSubjects/MED00290 polycyclic compounds Escherichia coli bacteria Humans AcademicSubjects/MED00740 AcademicSubjects/MED00230 Escherichia coli Infections Netherlands Original Research |
Zdroj: | Journal of Antimicrobial Chemotherapy |
ISSN: | 1460-2091 |
Popis: | Introduction The human gut microbiota is an important reservoir of ESBL-producing Escherichia coli (ESBL-Ec). Community surveillance studies of ESBL-Ec to monitor circulating clones and ESBL genes are logistically challenging and costly. Objectives To evaluate if isolates obtained in routine clinical practice can be used as an alternative to monitor the distribution of clones and ESBL genes circulating in the community. Methods WGS was performed on 451 Dutch ESBL-Ec isolates (2014–17), including 162 community faeces and 289 urine and blood isolates. We compared proportions of 10 most frequently identified STs, PopPUNK-based sequence clusters (SCs) and ESBL gene subtypes and the degree of similarity using Czekanowski’s proportional similarity index (PSI). Results Nine out of 10 most prevalent STs and SCs and 8/10 most prevalent ESBL genes in clinical ESBL-Ec were also the most common types in community faeces. The proportions of ST131 (39% versus 23%) and SC131 (40% versus 25%) were higher in clinical isolates than in community faeces (P |
Databáze: | OpenAIRE |
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