Popis: |
The precursors of TCRαβ+ CD8αα+ intraepithelial lymphocytes (IEL) arise in the thymus through a complex process of agonist selection. We and others have shown that the pro-apoptotic protein, Bim, is critical to limit the number of thymic IEL precursors (IELp), as loss of Bim at the CD4+ CD8+ double positive (DP) stage of development drastically increases IELp. The factors determining this cell death versus survival decision remain largely unknown. Here, we used CD4CreBcl2(f/f) mice to define the role of the anti-apoptotic protein, Bcl-2, and CD4CreBcl2(f/f)Bim(f/f) mice to determine the role of Bcl-2 in opposing Bim to promote survival of IELp. First, in wild-type mice, we defined distinct sub-populations within PD-1+ CD122+ IELp, based on their expression of Runx3 and α4β7. Co-expression of α4β7 and Runx3 marked IELp that were most dependent upon Bcl-2 for survival. Importantly, the additional loss of Bim restored Runx3+ α4β7+ IELp showing that Bcl-2 antagonizes Bim to enable IELp survival. Further, the loss of thymic IELp in CD4CreBcl2(f/f) mice also led to a dramatic loss of IEL in the gut and the additional loss of Bim restored gut IEL. The loss of gut IEL was due to both reduced seeding by IELp from the thymus as well as a requirement for Bcl-2 for peripheral IEL survival. Together, these findings highlight subset-specific and temporal roles for Bcl-2 in driving the survival of TCRαβ+ CD8αα+ IEL and thymic IELp. |