Genetic regulation of mitotic competence in G

Autor:	Kenichi, Sajiki, Yuria, Tahara, Lisa, Uehara, Toshio, Sasaki, Tomáš, Pluskal, Mitsuhiro, Yanagida
Rok vydání:	2018
Předmět:	Cell Nucleus Gene Expression Regulation Fungal Schizosaccharomyces Autophagy Metabolome Mitosis SciAdv r-articles Schizosaccharomyces pombe Proteins Resting Phase Cell Cycle Molecular Biology Cells Cultured Research Articles Research Article
Zdroj:	Science Advances
ISSN:	2375-2548
Popis:	Among 85 genes that maintain mitotic competence in quiescent eukaryotic cells, Nem1 protects nuclei from autophagy by regulating lipin. Quiescent (G0 phase) cells must maintain mitotic competence (MC) to restart the cell cycle. This is essential for reproduction in unicellular organisms and also for development and cell replacement in higher organisms. Recently, suppression of MC has gained attention as a possible therapeutic strategy for cancer. Using a Schizosaccharomyces pombe deletion-mutant library, we identified 85 genes required to maintain MC during the G0 phase induced by nitrogen deprivation. G0 cells must recycle proteins and RNA, governed by anabolism, catabolism, transport, and availability of small molecules such as antioxidants. Protein phosphatases are also essential to maintain MC. In particular, Nem1-Spo7 protects the nucleus from autophagy by regulating Ned1, a lipin. These genes, designated GZE (G-Zero Essential) genes, reveal the landscape of genetic regulation of MC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::794a55bfbd68338118c5913d2099437a https://pubmed.ncbi.nlm.nih.gov/30116786 Zobrazit plný text záznamu