| Autor: |
H S, Smith, M E, Lippman, A J, Hiller, M R, Stampfer, A J, Hackett |
| Rok vydání: |
1985 |
| Předmět: |
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| Zdroj: |
Journal of the National Cancer Institute. 74(2) |
| ISSN: |
0027-8874 |
| Popis: |
Epithelial cells were isolated and cultured from a number of human mammary specimens of both cancerous and noncancerous origin. Doxorubicin (Dx) sensitivity was measured at second passage with the use of a highly efficient clonogenic assay. For 23 different tumor specimens derived from patients without previous chemotherapy, the drug concentrations required to kill 50% of the cells varied approximately 35-fold. In contrast, for 11 tumor specimens from patients who relapsed after regimens containing Dx, the drug concentration for 50% survival varied only fivefold and the dose-response curves for these specimens clustered at the more resistant end of the spectrum. A wide range of sensitivities was also observed among 13 noncancerous mammary specimens; however, tumor tissue and noncancerous tissue from the same donor were similar. When cultures were subjected to drug incubation periods of 1 and 4 hours, dose-response curves were superimposable when plotted as a function of drug concentration multiplied by time. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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