Expression of the T-cell activation antigen, OX-40, identifies alloreactive T cells in acute graft-versus-host disease
| Autor: | T V, Tittle, A D, Weinberg, C N, Steinkeler, R T, Maziarz |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
CD4-Positive T-Lymphocytes
Graft vs Host Disease Cell Separation Receptors OX40 Lymphocyte Activation Lymphocyte Depletion Receptors Tumor Necrosis Factor Rats Tumor Necrosis Factor Receptor Superfamily Member 7 Up-Regulation Rats Inbred Lew T-Lymphocyte Subsets Radiation Chimera Animals Transplantation Homologous Female Rats Inbred BUF Biomarkers Bone Marrow Transplantation |
| Zdroj: | Blood. 89(12) |
| ISSN: | 0006-4971 |
| Popis: | The OX-40 molecule is expressed on the surface of recently activated T lymphocytes. The presence of OX-40 on CD4+ T cells was analyzed in a rat haplo-identical (parental --F1) bone marrow transplant model of acute graft-versus-host disease (aGVHD). Increased numbers of activated CD4+ T cells that expressed the OX-40 antigen were detected in peripheral blood soon after transplantation before the earliest sign of disease. The peak of OX-40 expression occurred 12 days posttransplantation with a range of 18% to 36% of circulating T cells and remained 10-fold above background, never returning to baseline. A slight increase in OX-40 expression (range, 1% to 6%) was also detected on peripheral blood lymphocytes from control syngeneic F1 --F1 recipients. OX-40+ T cells were isolated from spleen, skin, lymph node, and liver tissue of rats undergoing aGVHD, but not in syngeneic transplants. OX-40+ T cells isolated from these tissues were of donor origin and were shown to be alloreactive. These data raise the possibility of using the OX-40 antibody to detect and deplete selectively the T cells that cause aGVHD. |
| Databáze: | OpenAIRE |
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