| Autor: |
Diana C, Koester, Morgan A, Maly, Sarah, Putman, Katie L, Edwards, Karen, Meeks, Adrienne E, Crosier |
| Rok vydání: |
2022 |
| Zdroj: |
Animals : an open access journal from MDPI. 12(7) |
| ISSN: |
2076-2615 |
| Popis: |
Cheetahs have been the subject of reproductive study for over 35 years, yet steroid hormone activity remains poorly described after ovulation. Our objective was to examine and compare fecal progestagen (fPM), estrogen (fEM), and glucocorticoid (fGM) metabolite concentrations post-ovulation in pregnant and non-pregnant animals to better understand female physiology (1) during successful pregnancy, (2) surrounding frequent non-pregnant luteal phases, and (3) after artificial insemination (AI) to improve the low success rate. Secondarily, the authors also validated a urinary progestagen metabolite assay, allowing pregnancy detection with minimal sample collection. Fecal samples were collected from 12 females for ≥2 weeks prior to breeding/hormone injection (the PRE period) through 92 days post-breeding/injection. Samples were assessed for hormone concentrations using established enzyme immunoassays. Urine samples were collected for 13 weeks from 6 females after natural breeding or AI. There were no differences among groups in fGM, but in pregnant females, concentrations were higher ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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