| Autor: |
Shu-Juan, Liu, Wei-Guo, Zheng, Zong-Ling, Ji, Su-Min, Chen, Xin-Hai, Zhang, Li-Jun, Yang |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 19(2) |
| ISSN: |
1007-8738 |
| Popis: |
To clone the PID domain of human DOC-2 (nDOC-2) and express it in E.coli DH5alpha.The cDNA fragment encoding the PID domain of nDOC-2 was amplified by RT-PCR from normal human ovarian tissue and cloned to pUC19. The DNA fragment from the pUC19-nDOC-2 digested with BamH I and EcoR I was ligated to the BamH I/EcoR I digested prokaryotic expression vector pGEX-4T-1.The expression of fusion protein was induced with IPTG and the expressed product was identified by SDS-PAGE.(1)The sequencing and endonucleases digestion analysis showed that the fragment of nDOC-2 gene was insert into vectors pUC19 and pGEX-4T-1;(2)SDS-PAGE showed the nDOC-2 gene had been expressed in E.coli DH5alpha.The PID domain of nDOC-2 was expressed successfully in prokaryote, which makes preparation for further researching the function of DOC-2 and preparing antibodies to DOC-2 protein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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