| Popis: |
Ion-sensitive microelectrodes were used to measure Cl(-) and H(+) activities in the cytoplasm of the unicellular green alga Eremosphaera viridis de Bary. In the light, cytoplasmic Cl(-) activity was 2.2 millimolar at most and cytoplasmic H(+) activity was about 5.4.10(-8) molar (pH 7.3). Darkening resulted in a permanent increase of the Cl(-) activity to 3.2 millimolar and in a transient acidification, which was compensated within 3 to 5 minutes. Switching light on again decreased the Cl(-) activity to the light level (2.2 millimolar). Simultaneously, a transient alkalization of the cytoplasm was observed. The transient character of the light-dependent pH changes was probably caused by pH-stat mechanisms, whereas the light-dependent Cl(-) activity changes were compensated to a much smaller degree. Studies with different inhibitors (3-(3,4-dichlorophenyl)-1, 1-dimethylurea, piretanide, venturicidin) indicated a direct relation between the light-driven H(+) flow across the thylakoid membrane and the observed light-dependent Cl(-) and H(+) activity changes in the cytoplasm. It is suggested that light-driven H(+) flux across the thylakoid membrane was in part electrically compensated by a parallel Cl(-) flux. The resulting Cl(-) and H(+) activity changes in the stroma were compensated by Cl(-) and H(+) fluxes across the chloroplast envelope giving rise to the observed Cl(-) and H(+) activity changes in the cytoplasm. |
