| Autor: |
Thomas, Pauwelyn, Richard, Stahl, Lakyn, Mayo, Xuan, Zheng, Andy, Lambrechts, Stefan, Janssens, Liesbet, Lagae, Veerle, Reumers, Dries, Braeken |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Biomedical optics express. 9(4) |
| ISSN: |
2156-7085 |
| Popis: |
The high rate of drug attrition caused by cardiotoxicity is a major challenge for drug development. Here, we developed a reflective lens-free imaging (RLFI) approach to non-invasively record in vitro cell deformation in cardiac monolayers with high temporal (169 fps) and non-reconstructed spatial resolution (352 µm) over a field-of-view of maximally 57 mm2. The method is compatible with opaque surfaces and silicon-based devices. Further, we demonstrated that the system can detect the impairment of both contractility and fast excitation waves in cardiac monolayers. Additionally, the RLFI device was implemented on a CMOS-based microelectrode array to retrieve multi-parametric information of cardiac cells, thereby offering more in-depth analysis of drug-induced (cardiomyopathic) effects for preclinical cardiotoxicity screening applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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