| Autor: |
Jian-Yun, Li, Xin, Xiong, Dian-Wen, Wang, Xu-Yan, Zhang, Can, Huang, Ling-Li, Zou, Cai-Feng, Zheng, Xin, Chen, Chuan-Qing, Tu |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 30(6) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the effect and mechanism of artesunate (ARTS) combined with cytarabine(Ara-C) and/or daunorubicin (DNR) on the proliferation and apoptosis of MV4-11 human mixed-lineage leukemia rearranged（MLL-r） acute myeloid leukemia (AML) cell line.CCK-8 assay was used to detect the proliferation effect of individual or in combination of ARTS, DNR, Ara-C on MV4-11 cells. The ICThe inhibition effect of ARTS, Ara-C and DNR on the proliferation of MV4-11 were all dose-dependently (The in vitro study showed that tri-combination of ARTS+Ara-C+DNR and bi-combination of ARTS+Ara-C could inhibit the proliferation and promote apoptosis of MV4-11 cell line. The inhibition effect of these two combinations were significantly superior to that of the traditional Ara-C+DNR treatment. The mechanism underlying this finding may be identified by the down regulation of Caspase-3, while no altered expression was observed of Caspase-9, DR4 and DR5.青蒿酯联合阿糖胞苷±柔红霉素对MLL基因重排白血病细胞株MV4-11凋亡的影响及其机制研究.探讨青蒿酯（ARTS）联合柔红霉素（DNR）±阿糖胞苷（Ara-C）对MLL基因重排（MLL-r）急性髓系白血病(AML)细胞株MV4-11细胞增殖、凋亡的影响及作用机制.采用CCK-8法检测ARTS、DNR、Ara-C单药及联用对MV4-11细胞增殖率及计算单药的ICARTS、Ara-C、DNR均呈浓度依赖性抑制MV4-1l细胞增殖(体外研究显示ARTS+DNR+Ara-C、ARTS+Ara-C方案协同抑制MV4-11细胞增殖和诱导MV4-11细胞凋亡作用均强于传统方案Ara-C+DNR，其作用机制可能是通过下调Caspase-3表达发挥作用,对Caspase-9、DR4、DR5的表达无影响. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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