Migratory activity of human glioma cell lines in vitro assessed by continuous single cell observation

Autor:	T, Demuth, N J, Hopf, O, Kempski, D, Sauner, M, Herr, A, Giese, A, Perneczky
Rok vydání:	2001
Předmět:	Adult Male Microscopy Video Brain Neoplasms Osmolar Concentration Temperature Glioma Hydrogen-Ion Concentration Kinetics Cell Movement Tumor Cells Cultured Homeostasis Humans Female Neoplasm Invasiveness Aged
Zdroj:	Clinicalexperimental metastasis. 18(7)
ISSN:	0262-0898
Popis:	A new migration assay, the time-lapse individual cell migration assay (TIM-assay), was developed, which allows the observation of cells over 24 h under controlled conditions. Using this technique, the migratory behavior of 8 human glioblastoma cell lines in vitro was studied. Special features are simultaneous documentation of migratory parameters of individual cells, i.e., migration velocities and migration paths of individual cells. Migration velocity for cell populations of the same cell line ranged from 0 to 24 microm/h. The migration paths were examined for being directional. Two thirds of all cells showed directional migration. Migration paths were further classified according to visual judgements for being linear, oscillating or mixed. The migration index had a mean of 91%. The presented TIM-assay allows the assessment of several new parameters. that may be useful to identify subgroups of gliomas with different biological characteristics.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::773fae6560416755e3292624a984eb57 https://pubmed.ncbi.nlm.nih.gov/11688964 Zobrazit plný text záznamu Full text from SpringerLink