More antitumor efficacy of the PI3K inhibitor GDC-0941 in breast cancer with PIK3CA mutation or HER2 amplification status in vitro
| Autor: | Jie, Zheng, Huan, Wang, Jia, Yao, Xianjin, Zou |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Sulfonamides
Wound Healing Indazoles Class I Phosphatidylinositol 3-Kinases Receptor ErbB-2 Blotting Western Apoptosis Breast Neoplasms Cell Cycle Proteins Phosphatidylinositol 3-Kinases Cell Movement Cell Line Tumor Mutation Humans Female Indicators and Reagents Apoptosis Regulatory Proteins Cell Proliferation Phosphoinositide-3 Kinase Inhibitors Signal Transduction |
| Zdroj: | Die Pharmazie. 69(1) |
| ISSN: | 0031-7144 |
| Popis: | PIK3CA is probably the most commonly mutated kinase in several malignant tumors. Activation of class I phosphatidylinositol 3' kinase (PI3K) regulates tumor proliferation, survival, etc. This study sought to identify whether the pan-inhibitor has more antitumor efficacy in breast cancer cells with PIK3CA Mutation or HER2 amplification than basal-like cancer cells. The proliferation of breast cancer cells was measured by MTT assay in the presence of GDC-0941. Afterwards, we determined the visible changes in signaling in the PI3K/AKT/mTOR pathway. Finally, we examined GDC-0941 effects on cell cycle, apoptosis and motility. GDC-0941 exhibited excellent inhibition on three cell lines with PIK3CA mutation or HER2 amplification. In addition, GDC-0941 resulted in decreased Akt activity. GDC-0941 downregulated the key components of the cell cycle machinery, such as cyclin D1, upregulated the apoptotic markers and inhibited cell motility on three cell lines with PIK3CA Mutation or HER2 amplification. Antitumor activity of GDC-0941 treatment amongst tumor cell lines with PIK3CA mutation and HER2 amplification may have clinical utility in patients with these oncogenic alterations. |
| Databáze: | OpenAIRE |
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