AR-R15896AR blocks the early NMDA-induced loss of MAP2 in primary cortical cultures
| Autor: | N B, Laferrière, R, Tremblay, C L, Murray, R, Monette, D L, Brown, J P, Durkin, P, Morley |
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| Rok vydání: | 1999 |
| Předmět: |
Brain Chemistry
Cerebral Cortex Ionophores Pyridines Ionomycin Drug Evaluation Preclinical Nerve Tissue Proteins Receptors N-Methyl-D-Aspartate Potassium Chloride Rats Rats Sprague-Dawley Neuroprotective Agents Microscopy Fluorescence Animals Anticonvulsants Calcium Signaling Microtubule-Associated Proteins Cells Cultured |
| Zdroj: | Neurological research. 21(5) |
| ISSN: | 0161-6412 |
| Popis: | The low-affinity use-dependent N-methyl-D-aspartate (NMDA) receptor antagonist AR-R15896AR is neuroprotective in primary rat cortical cultures exposed to toxic concentrations of NMDA and reduces the magnitude of NMDA-triggered increases in [Ca2+]i. Here we show using fluorescence staining and measurements of microtubule-associated protein-2 (MAP2) levels, that AR-R15896AR inhibits the NMDA-induced loss of MAP2 that occurs within 2 min following NMDA exposure. Understanding the multiple, Ca(2+)-triggered intracellular events that occur following NMDA receptor stimulation is important to the development of safe and effective neuroprotective agents. |
| Databáze: | OpenAIRE |
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