Correlation between loss of PTEN expression and Akt phosphorylation in endometrial carcinoma
| Autor: | Y, Kanamori, J, Kigawa, H, Itamochi, M, Shimada, M, Takahashi, S, Kamazawa, S, Sato, R, Akeshima, N, Terakawa |
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| Rok vydání: | 2001 |
| Předmět: |
Tumor Suppressor Proteins
Statistics as Topic PTEN Phosphohydrolase Protein Serine-Threonine Kinases Immunohistochemistry Phosphoric Monoester Hydrolases Endometrial Neoplasms Phosphatidylinositol 3-Kinases Proto-Oncogene Proteins Humans Female bcl-Associated Death Protein Gene Silencing Phosphorylation Carrier Proteins Proto-Oncogene Proteins c-akt |
| Zdroj: | Clinical cancer research : an official journal of the American Association for Cancer Research. 7(4) |
| ISSN: | 1078-0432 |
| Popis: | The tumor suppressor PTEN acts as a lipid phosphatase, regulates the phosphatidylinositol 3-kinase (PI3K)/Akt-signaling pathway, and modulates cell cycle progression and cell survival. Somatic mutations of PTEN have been reported in a variety of cancers, especially in endometrial carcinoma. To clarify whether and how PTEN and the PI3K/Akt pathway relates to endometrial carcinoma, we examined the expression of those pathway-related proteins in patients with endometrial carcinoma. Of 103 endometrial carcinomas, 37 (36%) showed negative immunohistochemical staining of PTEN. Western blotting revealed that the expression of PTEN in PTEN-negative cases was significantly lower compared with that in positive cases. In contrast, phospho-Akt level in negative cases was significantly higher. We found a significant inverse correlation between PTEN and phospho-Akt (r = -0.796). The expression of phospho-Bad was greater in negative cases, suggesting that Bad might be a target for AKT: The present study demonstrates the phosphorylation of Akt accompanied by the loss of PTEN in clinical specimens of endometrial carcinomas. |
| Databáze: | OpenAIRE |
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