HLA-DQ8 transgenic mice lacking endogenous class II molecules respond to house dust allergens: identification of antigenic epitopes

Autor:	T, Neeno, C J, Krco, J, Harders, J, Baisch, S, Cheng, C S, David
Rok vydání:	1996
Předmět:	Mites Immunodominant Epitopes Molecular Sequence Data Dose-Response Relationship Immunologic Histocompatibility Antigens Class II Mice Transgenic Allergens Th1 Cells Mice Th2 Cells HLA-DQ Antigens Animals Amino Acid Sequence Antigens Dermatophagoides Lymph Nodes Cells Cultured Glycoproteins
Zdroj:	Journal of immunology (Baltimore, Md. : 1950). 156(9)
ISSN:	0022-1767
Popis:	We have introduced HLA-DQ8 (HLADQB*0302 and HLA-DQA*0301) genes into A beta 0 knockout mice. Transgenic animals were immunized with a whole body extract of Dermatophagoides pteronyssinus (Der p), one of the causative agents of house dust mite allergy. Transgenic mice expressing HLA-DQ8 genes elicited HLA-DQ8-restricted responses driven by CD4+ T cells. Synthetic-overlapping peptides representing a major allergen of house dust mite (Der p 2) were synthesized and used as immunogens. HLA-DQ8+ mice responded to three peptides: 8 (residues 61-80), 11 (residues 91-110), and 13 (residues 111-129). The mice produced IL-2, IL-4, and IL-6 response to Der p challenge, suggesting a mixed Th1/Th2 response. These mice represent a new model for studies of the immune basis of allergy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::768f0caeb4c28195a23938f90ab82ac1 https://pubmed.ncbi.nlm.nih.gov/8617940 Zobrazit plný text záznamu