Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene
| Autor: | Wang, Ju-Hua, Xue, Xiu-Heng, Zhou, Jie, Fan, Cai-Yun, Xie, Qian-Qian, Wang, Pan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
ATP-binding cassette transporter protein
Iron Cryptosporidium andersoni iron transportation Molecular Sequence Data cloning Protozoan Proteins Cryptosporidiosis Cryptosporidium Brief Communication Protein Structure Tertiary Mice Adenosine Triphosphate Animals Humans ATP-Binding Cassette Transporters Calcium Amino Acid Sequence Cloning Molecular Sequence Alignment |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 0023-4001 |
| Popis: | Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of Ca(2+), Mg(2+), K(+), and HCO3 (-) in IECs (P0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|