| Autor: |
J F, Cooper, M E, Weary, F T, Jordan |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
PDA journal of pharmaceutical science and technology. 51(1) |
| ISSN: |
1079-7440 |
| Popis: |
Limulus amebocyte lysate (LAL) is activated by bacterial endotoxins and certain glucans (beta-D-glucan, LAL-RM). The potential for conflicting inter-laboratory results for LAL tests exists because commercial LAL reagents are highly variable in response to LAL-reactive glucans. The nature of beta-D-glucan activation of LAL and means for rendering LAL non-responsive to glucan are reviewed to provide a background for resolving conflicting data. Kinetic LAL methods are particularly useful for screening materials potentially contaminated with glucan. The presence of beta-D-glucan in parenterals is uncommon and is likely limited to products exposed to microbial or cellulosic materials. A scheme is suggested for identifying LAL-reactive glucans and for LAL release-testing without glucan interference. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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