| Autor: |
Jana, Blahova, Kamila, Kruzikova, Klara, Hilscherova, Roman, Grabic, Jarmila, Halirova, Jana, Jurcikova, Tomas, Ocelka, Zdenka, Svobodova |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Neuro endocrinology letters. 29(5) |
| ISSN: |
0172-780X |
| Popis: |
The aim of this study was the assessment of the Svitava and Svratka rivers contamination by polycyclic aromatic hydrocarbons (PAH) using 1-hydroxypyrene (1-OHP) concentrations in fish bile as a biomarker.Levels of 1-OHP were determined by reverse phase HPLC with fluorescence detection. For valid assessment of bile accumulation levels, the 1-OHP concentration was normalized to the biliary protein content. The content of 1-OHP was correlated with the PAH content of river bottom sediments.The highest level of 1-OHP in fish bile (136.1 ng.mg-1 protein) was found at the locality Rajhradice, which is situated downstream of Brno. Also the greatest level of sum of PAHs was found at this locality (17.1 microg.g-1 dry mass). Significant positive correlation (por =0.05) between the level of 1-OHP and sum of PAHs in sediment was found only in case of sediments collected in the same month as fish samples (in June).Our results document that 1-OHP in fish bile is a suitable biochemical marker for the assessment of aquatic ecosystem contamination by PAHs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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