| Autor: |
C C, Chang, Y Y, Tsong, J D, Rone, S J, Segal, D, Chang, J, Leban, K, Folkers |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
Fertility and sterility. 36(5) |
| ISSN: |
0015-0282 |
| Popis: |
Antisera generated to the human chorionic gonadotropin beta-subunit (hCGbeta) have been shown not only to neutralize the biologic activity of hCG but also to cross-react with human luteinizing hormone (hLH). In an attempt to reduce such cross-reactivity, a peptide fragment analogous to the amino acid sequence of the carboxylterminal 45 residues (101-145) of the hCGbeta-subunit with alpha-aminobutyric acid substituting for cysteine at position 110 was synthesized and tested for ability to produce antibodies interacting with hCG. Antisera were generated in rabbits to a conjugate of this peptide with tetanus toxoid emulsified with Freund's complete adjuvant. Antibody titers and specificity were assessed by the double-antibody technique. The results show that the antisera to the synthetic hCGbeta fragment bound 125I-labeled hCG and did not cross-react with hLH in the radioimmunoassay system. Most importantly, the antisera effectively neutralized the biologic activity of hCG as determined by the rat uterine weight assay. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
