Differential expression of nucleoside diphosphate kinases (NDPK/NM23) during Xenopus early development
| Autor: | T, Ouatas, M, Sélo, Z, Sadji, J, Hourdry, H, Denis, A, Mazabraud |
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| Rok vydání: | 1998 |
| Předmět: |
Transcription
Genetic Xenopus Gene Expression Regulation Developmental Nuclear Proteins Cell Differentiation NM23 Nucleoside Diphosphate Kinases Immunohistochemistry Nucleoside-Diphosphate Kinase Protein Biosynthesis Animals RNA Messenger In Situ Hybridization Monomeric GTP-Binding Proteins Transcription Factors |
| Zdroj: | The International journal of developmental biology. 42(1) |
| ISSN: | 0214-6282 |
| Popis: | In Xenopus laevis, three nucleoside diphosphate kinase (NDPK) monomers have been described (NDPK X1, X2 and X3) (Ouatas et al., 1997). In eucaryotes, this kinase is known as a hetero- or homohexamer. Here, we examine the distribution of the enzyme and its different subunit mRNAs during oogenesis and early embryogenesis of Xenopus laevis, respectively by immunohistofluorescence and whole-mount in situ hybridization. These analyses show that NDPKs and their mRNAs are differentially distributed throughout the oocyte and early embryos with a high level of transcription in somites and brain. We emphasize two points. First, each mRNA displays a distinct subcellular localization in somites, suggesting a complex regulation of NDPK genes both at the transcriptional and translational level and a possible involvement of NDPK X2 homohexamers in the dorsal muscle differentiation. Second, in oocytes and early embryos, the proteins are mainly localized in the nucleus, suggesting a new mechanism for their nuclear import, since they do not possess any known nuclear import sequences. |
| Databáze: | OpenAIRE |
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