| Autor: |
Ying-mei, Zhang, Ying, Wang, Qian-mei, Xu, Bing-feng, Lv, Da-long, Ma |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences. 38(6) |
| ISSN: |
1671-167X |
| Popis: |
To develop a reporter gene system based on transient transfections with a NF-kappaB responsive reporter gene to detect the bioactivity of IL-1beta and IL-1 receptor antagonist.NF-kappaB reporter and Dual-Luciferase assays were applied to measure the bioactivity of IL-1beta and IL-1 receptor antagonist in mouse EL4 cells (some subclones of EL4 cells expressed high level of IL-1 receptor on cell surface). pNF-kappaB-luc and pRL-TK, used as an internal control, were co-transfected into EL4 cells and then the IL-1beta was added.The results indicated that IL-1beta was able to induce the expression of this luciferase, which could be blocked by IL-1 receptor antagonist. The optimal dose of IL-1beta was 5 microg/L in Dual-Luciferase assay, whose bioactivity can be effectively inhibited by IL-1ra at 50 microg/L.We have established a new method to detect the bioactivity of IL-1beta and IL-1 receptor antagonist, which can give repeatable results. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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