| Autor: |
C, Verret, S, Poussard, K, Touyarot, C, Donger, M, Savart, P, Cottin, A, Ducastaing |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Biochimica et biophysica acta. 1430(1) |
| ISSN: |
0006-3002 |
| Popis: |
In previous studies, we isolated and identified a mu-calpain-PKCalpha complex from rabbit skeletal muscle. At the same time we pointed out that an association between mu-calpain and PKCalpha could occur at the level of the plasma membrane of muscle cells, and that PKCalpha could thus be considered as a potential mu-calpain substrate. In the present study, using the mu-calpain-PKCalpha complex as a model, we report that mu-calpain is activated in the combined presence of physiological calcium concentrations (less than 1 microM) and phosphatidylserine. Furthermore our data also show that: (1) there exists a correlation between the appearance of autolyzed mu-calpain forms and PKCalpha hydrolysis which leads to the formation of PKMalpha; (2) in certain experimental conditions, autolyzed mu-calpain forms are able to hydrolyze PKMalpha independently of the presence of diacylglycerol. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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