Association between
| Autor: | Carolina Rosal Teixeira, de Souza, Marcelli Carolini Alves, Almeida, André Salim, Khayat, Emerson Lucena, da Silva, Paulo Cardoso, Soares, Luiz Cláudio, Chaves, Rommel Mario Rodríguez, Burbano |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
DNA Bacterial Male Epstein-Barr Virus Infections Herpesvirus 4 Human Human papillomavirus Microorganisms Adenocarcinoma Helicobacter Infections Bacterial Proteins Stomach Neoplasms Retrospective Study Humans Epstein-Barr virus Papillomaviridae Aged Neoplasm Staging Aged 80 and over Antigens Bacterial cagA Helicobacter pylori Coinfection Papillomavirus Infections Stomach Gastric adenocarcinomas Middle Aged bacterial infections and mycoses DNA Viral Female Gastric cancer Brazil |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 2219-2840 |
| Popis: | AIM To correlate Helicobacter pylori (H. pylori), Epstein-Barr virus (EBV) and human papillomavirus (HPV) with gastric cancer (GC) cases in Pará State, Brazil. METHODS Tissue samples were obtained from 302 gastric adenocarcinomas. A rapid urease test was used to detect the presence of H. pylori, and the presence of the cagA gene in the HP-positive samples was confirmed by PCR. An RNA in situ hybridization test designed to complement Eber1 RNA was used to detect the presence of EBV in the samples, and the L1 region of HPV was detected using nested PCR. Positive HPV samples were genotyped and analyzed for E6 and E7 viral gene expression. Infections were also correlated with the clinical and pathological characteristics of the patients. RESULTS The majority of the 302 samples analyzed were obtained from men (65%) aged 55 years or older (67%) and were classified as the intestinal subtype (55%). All three pathogens were found in the samples analyzed in the present study (H. pylori: 87%, EBV: 20%, HPV: 3%). Overall, 78% of the H. pylori-positive (H. pylori+) samples were cagA+ (H. pylori-cagA+), and there was an association between the cytotoxic product of this gene and EBV. Coinfections of H. pylori-cagA+ and EBV were correlated with the most advanced tumor stages. Although only 20% of the tumors were positive for EBV, infection with this virus was associated with distant metastasis. Only the HPV 16 and 18 strains were found in the samples, although no expression of the E6 and E7 oncoproteins was detected. The fundus of the stomach was the region least affected by the pathogens. CONCLUSION HPV was not involved in gastric tumorigenesis. Prophylactic and therapeutic measures against H. pylori and EBV may prevent the development of GC, especially the more aggressive forms. |
| Databáze: | OpenAIRE |
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