Inhibition of vascular endothelial growth factor-induced endothelial cell migration by ETS1 antisense oligonucleotides
| Autor: | Z, Chen, R J, Fisher, C W, Riggs, J S, Rhim, J A, Lautenberger |
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| Rok vydání: | 1997 |
| Předmět: |
Vascular Endothelial Growth Factor A
Gene Expression Endothelial Growth Factors Proto-Oncogene Protein c-ets-1 Cell Movement Proto-Oncogene Proteins Humans RNA Messenger Cells Cultured Lymphokines Membranes Proto-Oncogene Proteins c-ets Vascular Endothelial Growth Factors Oligonucleotides Antisense Urokinase-Type Plasminogen Activator Stimulation Chemical Drug Combinations Gene Expression Regulation Gelatin Tetradecanoylphorbol Acetate Proteoglycans Collagen Endothelium Vascular Laminin Cell Division Transcription Factors |
| Zdroj: | Cancer research. 57(10) |
| ISSN: | 0008-5472 |
| Popis: | Vascular endothelial growth factor (VEGF) increased the level of ETS1 mRNA in human umbilical vein endothelial cells (HUVEC) and human lung microvascular endothelial cells (HMVEC-L) over 5-fold. Protein levels were shown to increase concordantly. VEGF was also found to stimulate the invasiveness of endothelial cells as measured by migration through Matrigel- or gelatin-coated membranes. The VEGF-induced invasiveness was inhibited by ETS1 antisense oligonucleotides but not by a sense control. In addition, the ETS1 antisense oligonucleotides reduced the levels of ETS1 and urokinase-type plasminogen activator mRNAs. The antisense oligonucleotides directed against the ETS1 gene thus altered a cellular property of endothelial cells that is correlated with the ability of the cells to migrate through basement membranes. Together, these observations demonstrate a direct role for the ETS1 gene in angiogenesis. |
| Databáze: | OpenAIRE |
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