Allograft inflammatory factory-1. A cytokine-responsive macrophage molecule expressed in transplanted human hearts
| Autor: | U, Utans, W C, Quist, B M, McManus, J E, Wilson, R J, Arceci, A F, Wallace, M E, Russell |
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| Rok vydání: | 1996 |
| Předmět: |
Graft Rejection
Cytoplasm Base Sequence Sequence Homology Amino Acid Macrophages Myocardium Calcium-Binding Proteins Microfilament Proteins Molecular Sequence Data Gene Expression Macrophage Activation Rats DNA-Binding Proteins Tumor Cells Cultured Animals Heart Transplantation Humans Tissue Distribution Sequence Alignment DNA Primers |
| Zdroj: | Transplantation. 61(9) |
| ISSN: | 0041-1337 |
| Popis: | Allograft inflammatory factor-1 (AIF-1), a cytokine-responsive macrophage molecule, was originally identified and cloned from rat cardiac allografts with chronic cardiac rejection. We performed the present study to determine whether AIF-1 was also involved in the inflammatory response associated with human cardiac transplant rejection. AIF-1 gene transcripts were identified by the reverse-transcriptase polymerase chain reaction in endomyocardial biopsy specimens from human heart transplants and in macrophage cell lines. In the 441-base pair coding region of the human and rat cDNAs, the nucleotide sequences were 86% identical and the deduced amino acid sequences were 90% identical. Consistent with our studies in the rat. AIF-1 was selectively expressed in human macrophage-like cell lines, and immunostaining in human heart allografts localized the AIF-1 gene product to a subset of CD68+ macrophages in the interstitial and perivascular spaces. The parallels between rat and human AIF-1 expression suggest that AIF-1 may have a common effect on the function of activated macrophages in cardiac allografts. |
| Databáze: | OpenAIRE |
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