Integration site of human papillomavirus type-18 DNA in chromosome band 8q22.1 of C4-I cervical carcinoma: DNase I hypersensitivity and methylation of cellular flanking sequences

Autor:	M I, Gallego, D B, Zimonjic, N C, Popescu, J A, DiPaolo, P A, Lazo
Rok vydání:	1994
Předmět:	Virus Integration DNA Viral Papillomavirus Infections Carcinoma Squamous Cell Tumor Cells Cultured Humans Uterine Cervical Neoplasms Female Papillomaviridae Chromosomes Human Pair 8 HeLa Cells
Zdroj:	Genes, chromosomescancer. 9(1)
ISSN:	1045-2257
Popis:	The C4-I cell line derived from a non-keratinizing squamous cell carcinoma of the uterine cervix contains integrated human papillomavirus-18 DNA. Fluorescence in situ hybridization of C4-I cells demonstrated a single viral integration site at 8q22.1 on a derivative chromosome originating from an 8q;12q translocation. 8q22 is a site of chromosome fragility and is also recombinogenic in several human malignancies. DNase I hypersensitivity of the integration site was studied with a cellular flanking probe. A hypersensitive site was detected within 3 kb from viral DNA. The integration sites are undermethylated in C4-I and HeLa cells and fully methylated in tumor cell lines of other origin, such as lymphoid cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::74f44c838d66282d9b603980b0463f93 https://pubmed.ncbi.nlm.nih.gov/7507697 Zobrazit plný text záznamu Plný text