A developmental and tissue-specific enhancer in the mouse skeletal muscle acetylcholine receptor alpha-subunit gene regulated by myogenic factors
| Autor: | C A, Prody, J P, Merlie |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Transcriptional Activation Base Sequence Macromolecular Substances Muscles Recombinant Fusion Proteins Molecular Sequence Data Restriction Mapping Muscle Proteins Nuclear Proteins 3T3 Cells Phosphoproteins Transfection Cell Line Mice Enhancer Elements Genetic Gene Expression Regulation Sequence Homology Nucleic Acid Trans-Activators Animals Myogenin Receptors Cholinergic Chickens MyoD Protein |
| Zdroj: | The Journal of biological chemistry. 266(33) |
| ISSN: | 0021-9258 |
| Popis: | The expression of the nicotinic acetylcholine receptor (AChR) in vertebrate striated muscle is regulated both during development and in response to nerve-evoked muscle activity. To define DNA sequences necessary for the transcriptional regulation of the mouse alpha-subunit AChR gene, we have isolated and analyzed the alpha-gene 5'-flanking region. Primer extension and RNase protection analysis showed that transcription initiates at 2 major and 12 minor sites close to the translational initiation site. Using a series of plasmids in which segments of the 5'-flanking region were linked to the bacterial chloramphenicol acetyltransferase (CAT) gene, we have defined an 86-base pair enhancer sequence that is active in C2 myotubes but not in C2 myoblasts or NIH3T3 fibroblasts. This enhancer contains three putative binding sites for myoD1, and the 5'-upstream regions linked to CAT were transactivated by the muscle regulatory factors, myoD1, and myogenin. Transactivation by MRF4 differed with the specific alpha-subunit construct tested. Whereas the alpha-subunit CAT constructs containing both the homologous as well as the heterologous myosin light chain 1 promoter were transactivated by myoD1 and myogenin, only the constructs containing their homologous promoter were transactivated by MRF4. Thus, an 86-base pair sequence of the alpha-subunit gene contains the information necessary for developmental specificity and responsiveness to myogenic factors. |
| Databáze: | OpenAIRE |
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