| Autor: |
K S, Rysakova, V Iu, Novikov, V A, Mukhin, E M, Serafimchik |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia. 44(3) |
| ISSN: |
0555-1099 |
| Popis: |
Enzyme preparation exhibiting glycolytic activity yielding chitooligosaccharides along with N-acetyl-D-glucosamine was obtained from the red king crab (Paralithodes camtschaticus) hepatopancreas. The results of the analysis confirmed the presence of endo- and exochitinase activities in the preparation. HPLC showed that the hydrolysis products of chitin and chitosan did not contain D(+)-glucosamine, which is indicative of the absence of deacetylase and, apparently, exochitosanase activities. A comparison of the dependence of the enzyme preparation activity on temperature and pH of the incubation medium suggests that chitinase and protease activities are exhibited by different enzymes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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