Glutathione and Homoglutathione Synthetases of Legume Nodules. Cloning, Expression, and Subcellular Localization1
| Autor: | Moran, Jose F., Iturbe-Ormaetxe, Iñaki, Matamoros, Manuel A., Rubio, Maria C., Clemente, Maria R., Brewin, Nicholas J., Becana, Manuel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary Plants Medicinal Reverse Transcriptase Polymerase Chain Reaction Molecular Sequence Data Fabaceae Blotting Northern Plant Roots Glutathione Synthase Cell Compartmentation Cytosol Nitrogen Fixation Bacteroides Amino Acid Sequence Plastids Cloning Molecular Peptide Synthases Symbiosis Sequence Alignment Phylogeny Research Article Subcellular Fractions |
| Popis: | The thiol tripeptides glutathione (GSH) and homoglutathione (hGSH) are very abundant in legume root nodules and their synthesis is catalyzed by the enzymes gamma-glutamylcysteine synthetase (gammaECS), GSH synthetase (GSHS), and hGSH synthetase (hGSHS). As an essential step to elucidate the role of thiols in N(2) fixation we have isolated cDNAs encoding the three enzymes and have quantified the transcripts in nodules. Assay of enzyme activities in highly purified nodule organelles revealed that gammaECS is localized in the plastids, hGSHS in the cytosol, and GSHS in the cytosol and mitochondria. These results are consistent with sequence analyses. Subcellular fractionation of nodules also showed that bacteroids contain high thiol concentrations and high specific gammaECS and GSHS activities. Results emphasize the role of nodule plastids in antioxidant protection and in control of thiol synthesis, and suggest that plastids may be important in the stress response of nodules. Overall, our results provide further evidence that thiol synthesis is critical for nodule functioning. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|