| Autor: |
N Iu, Sakharova, I A, Kostanian, T N, Lepikhova, K A, Lepikhov, A M, Malashenko, E V, Navolotskaia, J, Tombran-Tink, V M, Lipkin |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Ontogenez. 33(3) |
| ISSN: |
0475-1450 |
| Popis: |
We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
