| Autor: |
Michael P, Ledbetter, Denis A, Malyshev, Floyd E, Romesberg |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1973 |
| ISSN: |
1940-6029 |
| Popis: |
The polymerase chain reaction (PCR) is a universal and essential tool in molecular biology and biotechnology, but it is generally limited to the amplification of DNA with the four-letter genetic alphabet. Here, we describe PCR amplification with a six-letter alphabet that includes the two natural dA-dT and dG-dC base pairs and an unnatural base pair (UBP) formed between the synthetic nucleotides dNaM and d5SICS or dTPT3 or analogs of these synthetic nucleotides modified with linkers that allow for the site-specific labeling of the amplified DNA with different functional groups. Under standard conditions, the six-letter DNA may be amplified with high efficiency and with greater than 99.9% fidelity. This allows for the efficient production of DNA site-specifically modified with different functionalities of interest for use in a wide range of applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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