Analysis of Paracoccidioides lutzii mitochondria: a proteomic approach

Autor: L, Casaletti, P S, Lima, L N, Oliveira, C L, Borges, S N, Báo, A M, Bailão, C M A, Soares
Rok vydání: 2016
Předmět:
Zdroj: Yeast (Chichester, England). 34(4)
ISSN: 1097-0061
Popis: The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analysed using a NanoUPLC-MS
Databáze: OpenAIRE
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