| Autor: |
I, Savini, I, D'Angelo, M, Annicchiarico-Petruzzelli, L, Bellincampi, G, Melino, L, Avigliano |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Anticancer research. 18(2A) |
| ISSN: |
0250-7005 |
| Popis: |
The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 +/- 9.8, SK-N-BE(2) 21.7 +/- 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 +/- 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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