| Autor: |
R D, Lane, A M, Kaplan, M J, Snodgrass, D J, Spriggs, A K, Szakal |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Journal of the National Cancer Institute. 72(4) |
| ISSN: |
0027-8874 |
| Popis: |
A rabbit antiserum was produced against a high-molecular-weight fraction (360,000) of murine Lewis lung carcinoma (LLC)-conditioned media. This fraction contained a factor that increased the rate of random migration (chemokinesis) of activated macrophages (M phi) in male C57BL/6J mice. The specificity of the antiserum was demonstrated by the ability of the antiserum to absorb the chemokinetic activity from tumor-conditioned media. Immunofluorescence studies demonstrated cell surface localization of antigenetically similar material on the LLC, Ehrlich ascites cells, MCA9/14 and MCA64/8 mouse fibrosarcomas, and MBT-2 mouse bladder carcinoma. The antiserum further indicated the presence of the chemokinetic factor (CKF) on the surface of peritoneal M phi previously exposed to tumor media. The CKF was observed on approximately 90% of the Corynebacterium parvum-activated M phi and of the M phi activated by maleic anhydride-divinyl ether copolymer (fraction 2), on 10% of the oyster glycogen-elicited M phi, and on 0% of the unstimulated M phi. these data support the concept that the CKF is a common surface marker of neoplastic cells and that it is bound by activated tumoricidal M phi. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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