Autor: |
Ping, Chen, Song, Nie, Jin-Yun, Xie, Song-Ping, Liang, Chong, Peng, Gui-Yuan, Li |
Rok vydání: |
2003 |
Předmět: |
|
Zdroj: |
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 35(7) |
ISSN: |
0582-9879 |
Popis: |
Guanidination of the epsilon-amino group of lysine-terminated tryptic peptides,accomplished with O-methylisourea hydrogen sulfate, converted lysine into homoarginine residues, improving detection in MALDI-MS. Then tryptic peptides were labeled with sulfonic acid groups at the N-termini using 3-sulfopropionic acid NHS-ester chemistry. The derivatives enhanced post-source decay (PSD) fragmentation signals and produced a spectrum containing only y-ions. This facilitated de novo peptide sequencing, so it is an important contribution to unambiguous protein identification in proteome research. This method was successfully applied in nasopharyngeal carcinoma(NPC) proteome study. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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