[Identification of protein spots in silver-stained two-dimensional gels based on chemically assisted fragmentation by MALDI-TOF-PSD mass spectrometry]

Autor:	Ping, Chen, Song, Nie, Jin-Yun, Xie, Song-Ping, Liang, Chong, Peng, Gui-Yuan, Li
Rok vydání:	2003
Předmět:	Silver Staining Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Molecular Sequence Data Proteins Electrophoresis Gel Two-Dimensional Amino Acid Sequence
Zdroj:	Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 35(7)
ISSN:	0582-9879
Popis:	Guanidination of the epsilon-amino group of lysine-terminated tryptic peptides,accomplished with O-methylisourea hydrogen sulfate, converted lysine into homoarginine residues, improving detection in MALDI-MS. Then tryptic peptides were labeled with sulfonic acid groups at the N-termini using 3-sulfopropionic acid NHS-ester chemistry. The derivatives enhanced post-source decay (PSD) fragmentation signals and produced a spectrum containing only y-ions. This facilitated de novo peptide sequencing, so it is an important contribution to unambiguous protein identification in proteome research. This method was successfully applied in nasopharyngeal carcinoma(NPC) proteome study.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::731019febcb7c8ca523172d9c33830b9 https://pubmed.ncbi.nlm.nih.gov/12883634 Zobrazit plný text záznamu