| Autor: |
Ping, Mao, Zheng-Gang, Zhao, Lan, Wang, Yu-Yu, Li, Mei-Rong, Li, Su-Jin, Zhou, Xin-Dan, Zhang, Yu, Wang, Fang-Hong, Li, Zi-Jian, Zhao |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 29(5) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the inhibitory effects of novel phosphodiesterase 4 inhibitor ZL-n-91 to the proliferation of leukemia cells L1210 and K562.CCK-8 method was used to detect the effect of ZL-n-91 to the proliferation of L1210 and K562 cells, and the proliferation rate, ICZL-n-91 showed a significant inhibitory effect to the proliferation of leukemia cells L1210 and K562 in a dose-dependent manner (P0.001). After treated by ZL-n-91, the leukemia cells L1210 and K562 in the S-phase in cell cycle decreased significantly compared with those in control group (P0.01). The apoptosis of leukemia cells L1210 and K562 could be induced by ZL-n-91 (P0.001), and the expression level of apoptosis related protein BAX significantly increased. In the animal experiment, the result showed that ZL-n-91 could significantly inhibit the growth of subcutaneously transplantation tumor (P0.05).The novel phosphodiesterase 4 inhibitor ZL-n-91 can effectively inhibit the proliferation of leukemia cells L1210 and K562, which has the potential of anti-leukemia drug development.新型磷酸二酯酶4抑制剂ZL-n-91对白血病细胞增殖抑制作用的研究.研究新型高选择性磷酸二酯酶4抑制剂ZL-n-91对白血病细胞L1210和K562增殖抑制的作用。.采用CCK-8法检测ZL-n-91对L1210和K562细胞增殖的影响，并计算增殖抑制率、半数抑制浓度（ICZL-n-91对白血病细胞L1210和K562具有显著的增殖抑制效果（P0.001），且抑制作用呈剂量依赖性。ZL-n-91处理后，白血病细胞L1210和K562细胞周期中S期的细胞减少，与对照组相比具有显著的统计学差异（P0.01）。ZL-n-91诱导L1210和K562白血病细胞发生凋亡（P0.001），凋亡相关蛋白BAX的表达显著增加。动物实验中，ZL-n-91可显著抑制白血病L1210皮下移植瘤的增殖（P0.05）。.新型磷酸二酯酶4抑制剂ZL-n-91能有效抑制白血病细胞L1210和K562的增殖，具有抗白血病药物开发的潜力。. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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