| Autor: |
K J, Prabahar, J P, Ferris |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life. 27(5-6) |
| ISSN: |
0169-6149 |
| Popis: |
The oligomerization of adenosine 5'-phosphoro-4-(dimethylamino)pyridinium (4-(CH3)2-NpypA) and diadenosine 5',5'-pyrophosphate (A5'ppA) (9:1) on Na(+)-montmorillonite was studied. The oligomers were isolated and analyzed by selective enzymatic hydrolyses and the oligomeric composition and the percent of 3',5'-phosphodiester linkages present in each fraction was determined. The longest oligomers formed (11-mers) are slightly shorter than those produced in the absence of A5'ppA (12-mers). Smaller amounts of A5'ppA are incorporated into the oligomers than in the ImpA/A5'ppA reaction. The regioselectivity of 3',5'-phosphodiester bond formation is comparable to that of the oligomerization of 4-(CH3)2NpypA alone. An explanation of these data is proposed and the possible effect of dinucleoside pyrophosphate on prebiotic RNA formation is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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